3MR0
Crystal Structure of Sensory Box Histidine Kinase/Response Regulator from Burkholderia thailandensis E264
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-04 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.265, 35.080, 71.348 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 30.578 - 1.493 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.19200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.578 | 1.540 |
High resolution limit [Å] | 1.493 | 1.500 |
Number of reflections | 46689 | |
<I/σ(I)> | 17.7 | 2.4 |
Completeness [%] | 97.2 | 75.6 |
Redundancy | 4.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Lithium sulfate, 0.1 M TRIS pH 8.5, 40 %v/v PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |