3MQZ
Crystal Structure of Conserved Protein DUF1054 from Pink Subaerial Biofilm Microbial Leptospirillum sp. Group II UBA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-04 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.967, 62.862, 81.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.015 - 1.300 |
R-factor | 0.1496 |
Rwork | 0.148 |
R-free | 0.17390 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.244 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 44135 | |
<I/σ(I)> | 17.1 | 4.6 |
Completeness [%] | 94.6 | 59.2 |
Redundancy | 8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M Sodium Formate, 20%PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |