3MQW
Crystal structure of a putative endoribonuclease L-PSP from Entamoeba histolytica with higher solvent content and an ordered N-terminal tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.000, 99.720, 106.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.800 |
| R-factor | 0.148 |
| Rwork | 0.147 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m4s |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.518 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.424 |
| Number of reflections | 81926 | |
| <I/σ(I)> | 14.29 | 2.7 |
| Completeness [%] | 98.6 | 90.9 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 290 | JCSG+ SCREEN CONDITION F2: 100MM CITRATE, 3150 MM AMMONIUM SULFATE, ENHIA.00579.A AT 26 MG/ML IN THE PRESENCE OF 2 MM SODIUM ORTHOVANADATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
