3MQK
Cbf5-Nop10-Gar1 complex binding with 17mer RNA containing ACA trinucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 105.696, 105.696, 243.925 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.378 - 2.800 |
| R-factor | 0.1903 |
| Rwork | 0.185 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ey4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.778 |
| Number of reflections | 20726 | |
| <I/σ(I)> | 48.8 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.6 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 303 | 120 mM magnesium acetate tetrahydrate, 50 mM MES PH 5.6, 20% 2-Methyl-2,4-pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
