3MQB
Crystal Structure of Ectodomain of BST-2/Tetherin/CD317 (C2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-04-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0717 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.650, 28.590, 94.510 |
Unit cell angles | 90.00, 111.43, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.25832 |
Rwork | 0.255 |
R-free | 0.31526 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mq7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.081 | 0.569 |
Number of reflections | 7240 | |
<I/σ(I)> | 12.5 | 1.9 |
Completeness [%] | 93.8 | 96.7 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | Crystals were grown at 25C using the microbatch under-oil method by mixing protein with crystallization buffer containing 100 mM HEPES, pH 7.5, 30% PEG 4000. 3-6% DMSO and 0.2-0.5 mM TCEP were used as additives for optimal crystal growth., EVAPORATION, temperature 298.0K |