3MQ9
Crystal Structure of Ectodomain Mutant of BST-2/Tetherin/CD317 Fused to MBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0091 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.502, 202.443, 107.277 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 42.470 - 2.800 |
| R-factor | 0.23341 |
| Rwork | 0.231 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Code:1PEB |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.470 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.116 | 1.000 |
| Number of reflections | 94908 | |
| <I/σ(I)> | 11.6 | 1 |
| Completeness [%] | 96.0 | 90.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 298 | Crystals were grown at 25C by using microbatch under oil by mixing protein with crystallization buffer containing 100 mM sodium acetate (pH 5.0), 200 mM NaCl, 20% PEG 6000. Micro batch under oil, pH 5.0, EVAPORATION, temperature 298.0K |
