3MQ4
Metabotropic glutamate receptor mGluR7 complexed with LY341495 antagonist
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-24 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.673, 131.159, 140.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.600 - 2.800 |
R-factor | 0.2635 |
Rwork | 0.262 |
R-free | 0.29330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ks9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.870 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 12809 | |
<I/σ(I)> | 46.29 | 2.12 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 20.0% PEG 3350, 0.2M NaH2PO4. protein at 5mg/mL plus 2mM LY341495, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |