3MQ4
Metabotropic glutamate receptor mGluR7 complexed with LY341495 antagonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.673, 131.159, 140.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.600 - 2.800 |
| R-factor | 0.2635 |
| Rwork | 0.262 |
| R-free | 0.29330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ks9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.870 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 12809 | |
| <I/σ(I)> | 46.29 | 2.12 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 20.0% PEG 3350, 0.2M NaH2PO4. protein at 5mg/mL plus 2mM LY341495, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
