3MQ3
Crystal structure of native bovine PDP1c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.274, 75.274, 172.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 2.800 |
| R-factor | 0.22706 |
| Rwork | 0.224 |
| R-free | 0.28433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.894 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.085 | 0.340 |
| Number of reflections | 14135 | |
| <I/σ(I)> | 20.6 | 7.6 |
| Completeness [%] | 96.9 | 97.9 |
| Redundancy | 4.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging drop | 7.5 | 298 | The protein solution was 20mg/mL in 10 mM Tris-HCl, pH 7.3, 50 mM NaCl, 1mM MnSO4, 2mM DTT, and 1mM benzamide. The well solution (mixed 1:1) contained 0.10M HEPES, pH 7.5 and 20% Jeffamine M-600., Hanging drop, temperature 298K |
