3MPY
Structure of EUTM in 2-D protein membrane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | P 6 |
Unit cell lengths | 69.164, 69.164, 28.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.290 - 2.000 |
R-factor | 0.22641 |
Rwork | 0.224 |
R-free | 0.28277 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2A1B (RESIDUES 2-90) |
RMSD bond length | 0.026 |
RMSD bond angle | 2.459 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.300 |
High resolution limit [Å] | 2.000 |
Number of reflections | 4477 |
<I/σ(I)> | 20.1 |
Completeness [%] | 81.1 |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2 M ammoniumsulfate, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |