3MP8
Crystal structure of Sgf29 tudor domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.760, 95.100, 114.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.140 - 1.920 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hsj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.986 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.096 | 0.234 |
| Number of reflections | 40594 | |
| <I/σ(I)> | 16.5 | 4.8 |
| Completeness [%] | 95.7 | 79.8 |
| Redundancy | 5.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 281 | 2.0M ammonium sulfate, 0.1M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
