3MP4
Crystal structure of Human lyase R41M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.490, 116.620, 86.980 |
| Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
| Resolution | 29.160 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cw6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.160 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 86402 | |
| <I/σ(I)> | 28.9 | 2.6 |
| Completeness [%] | 94.4 | 64.4 |
| Redundancy | 5.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | PEG8K, Hepes buffer, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
