3MOO
Crystal structure of the HmuO, heme oxygenase from Corynebacterium diphtheriae, in complex with azide-bound verdoheme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.609, 63.649, 78.520 |
Unit cell angles | 90.00, 130.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.710 |
R-factor | 0.18188 |
Rwork | 0.178 |
R-free | 0.21283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw0 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.469 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 37613 | |
<I/σ(I)> | 23.6 | 2.84 |
Completeness [%] | 96.2 | 82.8 |
Redundancy | 4.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.4 | 303 | 50mM MES (pH 5.4), 2.4M ammonium sulfate, 0.27M sodium bromide, 0.1 % (v/v) dioxane, VAPOR DIFFUSION, temperature 303K |