3MOH
The structure of rat cytosolic PEPCK mutant A467G in complex with phosphoglycolate and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.078, 119.520, 87.018 |
Unit cell angles | 90.00, 107.15, 90.00 |
Refinement procedure
Resolution | 35.930 - 2.100 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.484 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.163 | 0.067 | 0.735 |
Number of reflections | 68897 | ||
<I/σ(I)> | 6.7 | ||
Completeness [%] | 97.2 | 99.7 | 80.3 |
Redundancy | 7 | 7.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES PH 7.4,10 MM MNCL2, 10 MM GDP, hanging drop vapor diffusion, temperature 298K |