3MOH
The structure of rat cytosolic PEPCK mutant A467G in complex with phosphoglycolate and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.078, 119.520, 87.018 |
| Unit cell angles | 90.00, 107.15, 90.00 |
Refinement procedure
| Resolution | 35.930 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.163 | 0.067 | 0.735 |
| Number of reflections | 68897 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 97.2 | 99.7 | 80.3 |
| Redundancy | 7 | 7.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES PH 7.4,10 MM MNCL2, 10 MM GDP, hanging drop vapor diffusion, temperature 298K |
