3MO4
The crystal structure of an alpha-(1-3,4)-fucosidase from Bifidobacterium longum subsp. infantis ATCC 15697
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.610, 106.094, 122.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.934 - 1.901 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.20520 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.003 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.125 | 0.814 |
Number of reflections | 84888 | |
<I/σ(I)> | 13.5 | 1.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.8M succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |