3MNV
Crystal structure of the non-neutralizing HIV antibody 13H11 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 158.378, 158.378, 92.975 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.278 - 2.400 |
R-factor | 0.1826 |
Rwork | 0.181 |
R-free | 0.22780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mhh 1tjg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.075 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_271)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.278 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.058 |
Number of reflections | 51525 |
<I/σ(I)> | 11.4 |
Completeness [%] | 99.2 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | Qiagen AmSO4 suite, reservoir: 2.2 M ammonium sulfate, 0.2 M lithium nitrate. Drop: 0.5 uL protein + 0.5 uL reservoir., VAPOR DIFFUSION, temperature 298K |