3MNH
Human Carbonic Anhydrase II Mutant K170A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2008-02-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.645, 41.564, 72.750 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 20.780 - 1.650 |
R-factor | 0.15624 |
Rwork | 0.155 |
R-free | 0.18783 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tbt |
RMSD bond length | 0.027 |
RMSD bond angle | 2.142 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 29564 | |
<I/σ(I)> | 12.5 | 2.1 |
Completeness [%] | 98.4 | 98.1 |
Redundancy | 2.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.3M Sodium Citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |