3MN8

Structure of Drosophila melanogaster carboxypeptidase D isoform 1B short

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	97.020, 135.700, 141.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.700
R-factor	0.2116
Rwork	0.211
R-free	0.28170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h8l
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	2.700
Number of reflections	52035

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	8% PEG 4000, 0.2M KSCN, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K