3MMN
Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8123 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.142, 100.915, 80.139 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.200 |
R-factor | 0.17716 |
Rwork | 0.174 |
R-free | 0.23712 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mm4 |
RMSD bond length | 0.025 |
RMSD bond angle | 1.905 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 5.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 10328 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.05 | 293 | 0.1M MES, Amm. sulphate, pH 5.05, vapor diffusion, hanging drop, temperature 293K |