3MM0
Crystal structure of chimeric avidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.657, 124.791, 88.895 |
| Unit cell angles | 90.00, 114.45, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.22592 |
| Rwork | 0.222 |
| R-free | 0.30929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2avi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.420 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.630 | |
| Number of reflections | 42677 | |
| <I/σ(I)> | 14.3 | 1.3 |
| Completeness [%] | 98.1 | 90.1 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.3 | 293 | 20% Polyethylene glycol monomethyl ether 5000, 0.1M Tris (pH 8.3), and 9mM ammonium sulfate, microbatch, temperature 293K |
