3MLO
DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (Crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Detector technology | CCD |
| Collection date | 2009-08-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.750, 70.750, 601.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.710 - 3.010 |
| R-factor | 0.22387 |
| Rwork | 0.221 |
| R-free | 0.27778 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19172 | |
| <I/σ(I)> | 11.8 | 2.6 |
| Completeness [%] | 98.9 | 89.9 |
| Redundancy | 13.1 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 6% PEG4000, 200mM KCl, 100mM MES, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 6% PEG4000, 200mM KCl, 100mM MES, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
