3MLI
2ouf-ds, a disulfide-linked dimer of Helicobacter pylori protein HP0242
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-05 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.318, 75.648, 134.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.160 - 2.900 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OUF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.020 |
| High resolution limit [Å] | 2.800 | 2.900 |
| Rmerge | 0.096 | 0.537 |
| Number of reflections | 11588 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 13.2 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8.3% 2-propanol, 0.1 M MES pH 6.0, 0.32 M calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
