3MLH
Crystal structure of the 2009 H1N1 influenza virus hemagglutinin receptor-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.857, 74.065, 75.180 |
Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
Resolution | 39.720 - 2.090 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ruy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.127 | 0.373 |
Number of reflections | 25588 | |
<I/σ(I)> | 8.3 | 3.83 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 2000MME, Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |