3MLH
Crystal structure of the 2009 H1N1 influenza virus hemagglutinin receptor-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.857, 74.065, 75.180 |
| Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
| Resolution | 39.720 - 2.090 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ruy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.074 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.127 | 0.373 |
| Number of reflections | 25588 | |
| <I/σ(I)> | 8.3 | 3.83 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 2000MME, Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
