3MLF
Putative transcriptional regulator from Staphylococcus aureus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.776, 159.776, 69.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.300 - 2.600 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.659 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.300 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.146 | 0.790 |
Number of reflections | 27459 | |
<I/σ(I)> | 8.3 | 2.8 |
Completeness [%] | 97.5 | 99.4 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.1 M TRIS buffer, 1.4 M di-ammonium tartrate, 1 mM magnesium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |