3ML6

a complex between Dishevelled2 and clathrin adaptor AP-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	292.371, 98.137, 171.322
Unit cell angles	90.00, 121.97, 90.00

Refinement procedure

Resolution	49.070 - 3.500
R-factor	0.308
Rwork	0.308
R-free	0.33500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bw8 1fsh
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.112	0.774
Number of reflections	51429
<I/σ(I)>	11.3	1.5
Completeness [%]	99.3	97
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.7 M K/Na tartrate, 0.1 M sodium citrate, pH 5.5, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K