3ML3
Crystal structure of the IcsA autochaperone region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 62 |
| Unit cell lengths | 104.220, 104.220, 61.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.990 - 2.000 |
| R-factor | 0.19754 |
| Rwork | 0.196 |
| R-free | 0.21860 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 24863 | |
| <I/σ(I)> | 25.1 | 3.8 |
| Completeness [%] | 96.0 | 90.3 |
| Redundancy | 8.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 293 | 17% PEG 4000, 10% isopropanol, 0.1M sodium citrate, pH 6.0, EVAPORATION, temperature 293K |
