3MKZ
Structure of SopB(155-272)-18mer complex, P21 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 111.380, 47.120, 118.230 |
Unit cell angles | 90.00, 115.97, 90.00 |
Refinement procedure
Resolution | 50.070 - 2.980 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mkw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.980 |
Rmerge | 0.067 |
Number of reflections | 22277 |
<I/σ(I)> | 7.3 |
Completeness [%] | 99.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 400, Calcium chloride 200 mM, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |