3MKW
Structure of sopB(155-272)-18mer complex, I23 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.03 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 200.460, 200.460, 200.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.230 - 2.990 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | the FL SopB-18mer I23 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.050 | 0.448 |
| Number of reflections | 26809 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25.5% PEG 4000, 0.17 M lithium sulphate, 0.1 M Tris pH 8.0, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
