3MKM
Crystal structure of the E. coli pyrimidine nucleoside hydrolase YeiK (Apo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.601, 85.231, 90.060 |
| Unit cell angles | 66.65, 79.39, 85.30 |
Refinement procedure
| Resolution | 47.910 - 2.200 |
| R-factor | 0.18687 |
| Rwork | 0.185 |
| R-free | 0.22284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | YEIK PDB CODE 1Q8F |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.112 | 0.363 |
| Number of reflections | 58257 | |
| <I/σ(I)> | 10.8 | 3.2 |
| Completeness [%] | 94.8 | 94.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25% PEG 3350, 0.1M TRIS PH 8.5, 0.2M SODIUM CHLORIDE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
