3MKM
Crystal structure of the E. coli pyrimidine nucleoside hydrolase YeiK (Apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 |
Unit cell lengths | 45.601, 85.231, 90.060 |
Unit cell angles | 66.65, 79.39, 85.30 |
Refinement procedure
Resolution | 47.910 - 2.200 |
R-factor | 0.18687 |
Rwork | 0.185 |
R-free | 0.22284 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | YEIK PDB CODE 1Q8F |
RMSD bond length | 0.014 |
RMSD bond angle | 1.321 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.650 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.112 | 0.363 |
Number of reflections | 58257 | |
<I/σ(I)> | 10.8 | 3.2 |
Completeness [%] | 94.8 | 94.5 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25% PEG 3350, 0.1M TRIS PH 8.5, 0.2M SODIUM CHLORIDE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |