3MK7
The structure of CBB3 cytochrome oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.475, 279.933, 175.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.19074 |
| Rwork | 0.189 |
| R-free | 0.22341 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.162 | 0.830 |
| Number of reflections | 109170 | |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 98.2 | 99.9 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 20mM Tris(HCl), 0.1M NaCl, 0.058% alpha-UDM, 0.0125% decanoylsucrose, 0.018% alpha-DDM, 0.016M K3Fe(CN)6, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
