3MJ8
Crystal structure of HL4E10 Fab, a hamster Ab stimulatory for gammadelta T cells
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-12 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.836, 148.768, 68.798 |
Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.940 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HL4E10 LIGHT CHAIN SEQUENCE THREADED ONTO 1W72 HL4E10 HEAVY CHAIN SEQUENCE THREADED ONTO 2ARJ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.060 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.105 | 0.541 |
Number of reflections | 17012 | |
<I/σ(I)> | 7.7 | 1.7 |
Completeness [%] | 95.3 | 96.1 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10-12.5 % PEG 4000, 0.1 M NA- ACETATE, 0.2 M (NH4)2SO4, pH 4.60, VAPOR DIFFUSION, SITTING DROP, temperature 295K |