3MJ6
Crystal structure of the gammadelta T cell costimulatory receptor Junctional Adhesion Molecule-Like Protein, JAML
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.07812, 0.99984 |
Spacegroup name | P 43 |
Unit cell lengths | 61.946, 61.946, 82.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.190 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.21300 |
Structure solution method | MAD |
Starting model (for MR) | OBTAINED BY MAD PHASING OF A TA6BR12 SOAKED JAML CRYSTAL AND SUBSEQUENT MR INTO THE NATIVE HIGH RESOLUTION DATA |
RMSD bond length | 0.016 |
RMSD bond angle | 1.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.083 | 0.415 |
Number of reflections | 15924 | |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 295 | 3.25 M NA-FORMATE, 0.1 M IMIDAZOLE, pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 295K |