3MIW
Crystal Structure of Rotavirus NSP4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-05 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 |
Unit cell lengths | 63.463, 63.463, 123.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.382 - 2.500 |
R-factor | 0.2638 |
Rwork | 0.261 |
R-free | 0.29690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.508 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.382 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 | 0.187 |
Number of reflections | 16272 | |
<I/σ(I)> | 22.21 | 2.22 |
Completeness [%] | 95.1 | 74.6 |
Redundancy | 6.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% Dioxane v/v, 0.1 M MES, 1.6 M Ammonium Sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |