3MIW
Crystal Structure of Rotavirus NSP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-05 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 |
| Unit cell lengths | 63.463, 63.463, 123.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.382 - 2.500 |
| R-factor | 0.2638 |
| Rwork | 0.261 |
| R-free | 0.29690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.382 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.187 |
| Number of reflections | 16272 | |
| <I/σ(I)> | 22.21 | 2.22 |
| Completeness [%] | 95.1 | 74.6 |
| Redundancy | 6.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% Dioxane v/v, 0.1 M MES, 1.6 M Ammonium Sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
