3MII
Crystal structure of Y0R391Cp/HSP33 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 96.420, 96.420, 132.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.500 - 2.400 |
R-factor | 0.22337 |
Rwork | 0.222 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qvz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.946 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.630 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.118 | 0.443 |
Total number of observations | 105739 | |
Number of reflections | 45873 | |
<I/σ(I)> | 8.7 | 2.3 |
Completeness [%] | 97.3 | 99.4 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | 18% PEG 3000, 200mM (NH4)2SO4, 100mM tri-Sodium Citrate dihytrate, pH 5.6, VAPOR DIFFUSION, temperature 298K |