3MI6
Crystal structure of the alpha-galactosidase from Lactobacillus brevis, Northeast Structural Genomics Consortium Target LbR11.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97908, 0.97939, 0.91837 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.938, 158.818, 166.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.028 - 2.704 |
| R-factor | 0.1762 |
| Rwork | 0.173 |
| R-free | 0.24090 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.441 |
| Number of reflections | 161448 | |
| <I/σ(I)> | 22.8 | 4.8 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under paraffin oil | 7 | 291 | 10% PEG MME 5000, 5% Tacsimate, pH 7.0, 0.01M galactose, 0.1M HEPES pH 7.0, microbatch under paraffin oil, temperature 291K |
