3MH4
HtrA proteases are activated by a conserved mechanism that can be triggered by distinct molecular cues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 120.708, 120.708, 232.984 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.068 - 3.100 |
| R-factor | 0.2592 |
| Rwork | 0.257 |
| R-free | 0.30880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ky9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.936 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.092 | 0.463 |
| Number of reflections | 18503 | |
| <I/σ(I)> | 14.9 | 1.8 |
| Completeness [%] | 97.7 | 90 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 12% Isopropanol, 0.1M Tris, 12% PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, temperature 293K |
