3MGW
Thermodynamics and structure of a salmon cold-active goose-type lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.150, 103.150, 48.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.760 - 1.750 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 153l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.760 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.088 | 0.490 |
| Number of reflections | 19410 | |
| <I/σ(I)> | 11.3 | 1.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 43-45 % ammonium sulphate, 0.01M cobalt chloride, 0.1M MES , pH 6.25-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
