3MGJ
Crystal structure of the Saccharop_dh_N domain of MJ1480 protein from Methanococcus jannaschii. Northeast Structural Genomics Consortium Target MjR83a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.370, 65.395, 76.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.155 - 2.703 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25490 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.261 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.114 | 0.605 |
| Number of reflections | 10472 | |
| <I/σ(I)> | 23.5 | 3.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under paraffin oil | 4.2 | 291 | 2.21M lithium chloride, 0.1M sodium citrate, pH 4.2 , microbatch under paraffin oil, temperature 291K |
