3MGE
X-ray Structure of Hexameric HIV-1 CA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Collection date | 2009-06-30 |
Spacegroup name | P 6 |
Unit cell lengths | 90.313, 90.313, 56.527 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.281 - 1.900 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.908 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 20864 | |
<I/σ(I)> | 3.6 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% PEG 8,000 2% Tacsimate 100 mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |