3MG2

Crystal structure of the orange carotenoid protein Y44S mutant from cyanobacteria synechocystis sp. PCC 6803

Replaces:  3I1W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	83.188, 83.188, 87.974
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.542 - 2.653
R-factor	0.1974
Rwork	0.194
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I1V
RMSD bond length	0.003
RMSD bond angle	0.701
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6_288)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.740
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.129	0.037	0.687
Number of reflections	19757
<I/σ(I)>	7.1
Completeness [%]	100.0	99.9	100
Redundancy	5.6	5.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	12% PEG 4000, 100mM Sodium acetate, 2% Glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K