3MFY
Structural characterization of the subunit A mutant F236A of the A-ATP synthase from Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2008-12-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.535, 127.535, 106.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.750 - 2.350 |
R-factor | 0.22867 |
Rwork | 0.222 |
R-free | 0.27944 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vdz |
RMSD bond length | 0.018 |
RMSD bond angle | 1.720 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 35285 | |
<I/σ(I)> | 30.5 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.2 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 50%(v/v) MPD, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |