3MFT
CASK-4M CaM Kinase Domain, Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.87856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.943, 61.811, 101.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.21352 |
| Rwork | 0.210 |
| R-free | 0.26997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c0i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.248 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.568 |
| <I/σ(I)> | 11.3 | 1.3 |
| Completeness [%] | 96.9 | 84.4 |
| Redundancy | 4.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 12.5 % (v/v) ethylene glycol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
