3MFM
Crystal Structures and Mutational Analyses of Acyl-CoA Carboxylase Subunit of Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | .99 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.668, 220.961, 136.742 |
Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
Resolution | 38.824 - 2.380 |
R-factor | 0.2265 |
Rwork | 0.224 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.380 |
Rmerge | 0.100 |
Number of reflections | 182058 |
<I/σ(I)> | 3.75 |
Completeness [%] | 99.0 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 0.1M Bis-TrispH 6.8, 10% MPD, 0.2M Ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |