3MFF
1F1E8hu TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2004-06-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.323, 74.016, 94.267 |
| Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20203 |
| Rwork | 0.200 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.200 | 0.243 |
| Number of reflections | 74855 | |
| <I/σ(I)> | 20.2 | 0.284 |
| Completeness [%] | 91.7 | 63.9 |
| Redundancy | 9.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.3mM (NH4)2HydrogenCitrate, 20%(w/v) PEG3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
