3MEZ
X-ray structural analysis of a mannose specific lectin from dutch crocus (crocus vernus)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.422, 98.154, 105.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.940 |
| R-factor | 0.18015 |
| Rwork | 0.178 |
| R-free | 0.22504 |
| Structure solution method | MIR |
| Starting model (for MR) | 1msa |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.142 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.098 | 0.330 |
| Number of reflections | 48106 | |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4M Sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
