3MEU
Crystal structure of SGF29 in complex with H3R2me2sK4me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.04 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.140, 65.483, 106.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.660 - 1.280 |
R-factor | 0.20582 |
Rwork | 0.205 |
R-free | 0.22774 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3me9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.710 |
High resolution limit [Å] | 1.280 |
Number of reflections | 88614 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20-28% PEG3350, 0.1M Bis-Tris pH 5.5, vapor diffusion, Sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP |