3MEM
Crystal structure of a Putative signal transduction protein (Maqu_0641) from MARINOBACTER AQUAEOLEI VT8 at 2.25 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-24 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97911 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 148.670, 53.070, 69.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.126 - 2.250 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.563 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.126 | 32.126 | 2.310 |
High resolution limit [Å] | 2.250 | 10.060 | 2.250 |
Rmerge | 0.068 | 0.017 | 0.491 |
Number of reflections | 26590 | 528 | 3372 |
<I/σ(I)> | 9.39 | 38.1 | 1.5 |
Completeness [%] | 97.9 | 93 | 89.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 0.2000M NaFormate, 20.0000% PEG-3350, No Buffer pH 7.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |