3MEJ

Crystal structure of putative transcriptional regulator ywtF from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR736

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97901 0.97925 0.96791
Spacegroup name	P 41 21 2
Unit cell lengths	66.210, 66.210, 141.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.458 - 2.491
R-factor	0.2126
Rwork	0.206
R-free	0.27030
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.238
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge		0.469
Number of reflections	9933
<I/σ(I)>	44.7	7.3
Completeness [%]	100.0	100
Redundancy	20.4	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: PEG3350 19%, MgCl 0.2M HEPES 7.5 10mM EDTA drop plate , VAPOR DIFFUSION, HANGING DROP, temperature 293K