3MEB
Structure of cytoplasmic aspartate aminotransferase from giardia lamblia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.580, 101.150, 81.530 |
| Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
| Resolution | 47.810 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cst modified with CCP4 program CHAINSAW |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.091 | 0.336 |
| Number of reflections | 71707 | |
| <I/σ(I)> | 9.85 | 3 |
| Completeness [%] | 95.8 | 87.3 |
| Redundancy | 2.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | HR INDEX SCREEN G3: 20% PEG 3350, 200MM LI2 SULPHATE, 100MM BISTRISPROPANE, GILAA.01471.A AT 35 MG/ML, PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
