3MDU
The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-Guanidino-L-Glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 133.208, 133.208, 124.835 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.426 - 1.400 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.053 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.426 |
High resolution limit [Å] | 1.400 |
Rmerge | 0.089 |
Number of reflections | 102752 |
Completeness [%] | 94.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1M Sodium malonate, 0.1M Hepes, 0.5% Jeffamine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |