3MDP
Crystal structure of a Putative Cyclic nucleotide-binding protein (Gmet_1532) from Geobacter metallireducens GS-15 at 1.90 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-24 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97922,0.97898 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 95.862, 95.862, 35.485 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.520 - 1.900 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.511 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.520 | 28.520 | 1.970 |
High resolution limit [Å] | 1.900 | 4.080 | 1.900 |
Rmerge | 0.082 | 0.025 | 0.814 |
Number of reflections | 13586 | 2527 | 2567 |
<I/σ(I)> | 13.29 | 44.3 | 1.7 |
Completeness [%] | 99.8 | 99.5 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 10.0000% PEG-6000, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |